Tuesday, 10 May 2011

Lab 1


-Method
-Results
-analysis
-photo/video
In this practical, we we're asked to investigate the effects of sugar with yeast on cellular respiration.

Materials: 

  • test tubes (5)
  • weighting boat
  • weighing scale
  • sugar
  • yeast
  • spatula
  • breaker
  • test tube rack
  • thermometer
  • graduated cylinder
  • ruler

1.     Prepare the 0.5 grams of yeast using the weight boat as a place to add the yeast. Make sure when weighting the yeast that the tare mode is on to exclude the mass of the weighting boat
2.     Pour the yeast into the individual test tubes and place the test tubes on the test tube rack
3.     Use a graduated cylinder to measure out 10 ml of water into each of the test tubes with yeast
4.     Prepare the sugar on the weighing boats by increasing the amount of grams each time by 1.
5.     Prepare a hot water bath. Make sure that the water bath is at the 34 -27 degree range. 
6.     Once the water bath is ready, pour the sugar into each test tube IMPORTANT: MAKE SURE THAT THE SUGAR IS PUT AT THE SAME TIME FOR FAIR RESULTS.
7.     Record the height of the bubbles for every 5 – 10 mins.  

Changes to the method: We were suppose to use bromothymol blue and  hydrochloric acid as an indicator of cellular respiration, however the solution with the yeast did not respond to the bromothymol blue and the hydrochloric acid. Therefore the height of the bubbles was used as the results for cellular respiration

Faults to the experiment:  Just after 5 mins test tube 1 had a build up CO2 therefore making the cork of the test tube pop. This is because using too much sugar causing it to inhabitable.

Wednesday, 4 May 2011

Carbon Dioxide Consumption by Plants

The quick lab that we have done yesterday consisted of Bromothymol Blue Solution. This solution enabled us to determine wether or not the plant that was submerded into the water was doing photosysnthesis. There were two different controls one for light and one for dark and the other two that had a plant inside them. THe Bromothymol Blue and hydrocholric acid was an indication of color, when the liquid turns to yellow it becomes acidic which means that the plant did more cellular respiration and when the liquid turns green it mean that the plant did photosynthesis

The results were unexcpected. the opposite thing happened with the experiement. The plant with sunlight is supposed to be green then the one that does not have any sunlight at all. This meant that there has been an experiemental error with the procedure.